Cellular and Pharmacodynamic Imaging Facility
TMRM -Labeled Human Fibroblast Cell (Membrane Potential Image)
The Facility:
The imaging facility, run under the auspices of the Department of Pharmaceutical Sciences in the School of Pharmacy at Campbell University, is located on the third floor of the Riddle Pharmacy Building.
The Systems:
An Olympus FluoView confocal microscope system is configured about and IX-70 inverted microscope with computer control. Three different visible wavelength lasers (488, 543, and 633 nm) are used to excite fluorophores for the confocal microscope. The system has the capability for two fluorescent channels and one transmitted light channel. The microscope is equipped for DIC and fluorescence in the confocal mode and is capable of brightfield, phase, DIC and fluorescence in non-confocal operation. Long working distance water immersion lenses are also available. A color CCD camera is also attached for separate computer interfaced imaging with its own extensive software.
An Olympus IX-71 microscope equipped for phase contrast and fluorescent imaging with a cooled CCD camera for digital imaging and computer control is available in the facility. Digital imaging can be viewed using the Olympus Microsuite™ imaging software. Fluorescent excitation can be done with filter sets for UV and for fluorescein and rodamine type fluorescent probes.
A Hitachi F-4500, computer-controlled, fluorometer equipped with cell stirring and thermostatic temperature control is available in the facility. Both excitation and emission spectra can be obtained and stored digitally. Time-based and synchronous scans can also be performed. Extensive data analysis including 3-D contour plots can be done. Rapid scan features are also available.
A Hitachi 3010 UV/VIS, computer controlled, double beam spectrophotometer equipped with cell stirring and thermostatic temperature control is available in the facility. Time base and wavelength-based scans are possible. Rapid wavelength scans and kinetic measurements can be made on this instrument.
Imaging workstations are available to users of the facility to analyze images acquired on any of the imaging systems. The Olympus Fluoview, Microsuite™, Hitachi UVsolutions and FL Solutions software packages are available for analysis of data generated in the facility.
A Microm HM 550 cryostat for tissue sectioning is available in the facility for preparation of specimens to be imaged.
The Purpose:
The facility is intended to support cellular, pharmacological, and pharmaceutics research of both academic and industrial interest using a number of digital imaging technologies.
Support:
Funding support from the North Carolina Biotechnology Center in a grant to Dr. Chazotte has made possible the expansion of the facility.
Contact:
Dr. Brad Chazotte, Director
Department of Pharmaceutical Sciences
008 L.H. Campbell Hall
School of Pharmacy, Campbell University
Buies Creek, N.C. 27506
Phone: (910) 893-1816 Email: chazotte@mailcenter.campbell.edu
